Frequently Asked Questions

How long do I spin Blood?

For general blood processing, we recommend 10-15 minutes at 3300rpm. Please consult the protocol for your procedure for more detailed information.

How do I balance my centrifuge rotor?

                The centrifuge should be operated with all buckets installed (even if you are only spinning 2 test tubes).

Make sure that the centrifuge samples are ‘cross balanced’. (This means that the sample (total test tube + sample weight) across from each other on the rotor should have the same weight).

The correct loading configuration should be 2 test tubes only, 4 test tubes only (cross formation), or all 8 positions loaded. A 6-tube configuration will cause the rotor to be off balance.

 

Will the test tubes I use fit in Champion Centrifuges?

                E-33, E-33-12V, F-33D,  S-33:

S-50 & S-50D

How do I find proper settings of the centrifuge for my protocol?

Please consult the protocol for your procedure. This information is usually given in RCF, please refer to the RPM-RCF conversion document for your specific centrifuge model.

If you are using a test tube that contains coagulant or reagents, please consult the directions given by the manufacturer. This information is usually given in RCF, please refer to the RPM-RCF conversion document for your specific centrifuge model

What is the maximum power the centrifuges exerts?

E-33-110V : 60W

E-33-220V: 33W

E-33-12V: 18W

F-33D-110V: 63W

F-33D-220V: 51W

S-33-110V: 112W

S-33-220V: 71W

S-50D-110V: 188W

S-50D-220V: 188W

               

What kind of fuse does the centrifuge use?

                T3.15AL250V (size: 5 x 20mm)

E-33-12V centrifuge does not have an external fuse

My E-33/E-33-12V/F-33D centrifuge comes with small concave rubber paddings. What are these for?

The small concave rubber paddings are intended to be inserted into the bottom of the 15ml rotor buckets. These can help alleviate pressure between the test tube and the rotor buckets. You do not have to use these, especially if you are using centrifuge grade test tubes. We recommend to always use centrifuge grade test tubes in Ample Scientific centrifuges.